Practice question

A typical SPR cycle comprises baseline, association, dissociation, and regeneration. During association, analyte injected binds ligand, signal rises. When analyte injection stops and running buffer continues, free analyte concentration near surface drops to zero, preventing rebinding and favoring net complex decay. Bound analyte detaches according to off-rate kinetics, causing gradual decline in resonance units. Dissociation phase therefore reports kinetic stability of complex. Ligand addition occurs earlier, binding occurs in association, regeneration with harsh buffer follows after to strip remaining bound material for next cycle.