Practice question
Question
The formula for Rf (retention factor) is:
Explanation
Retention factor, Rf, also termed relative mobility, quantifies migration relative to solvent or tracking dye front. It is calculated as distance traveled by molecule of interest from origin (X) divided by distance traveled by dye front or solvent front from origin (Z). Rf values range 0 to 1 and are dimensionless, allowing comparison across gels. X/Z accounts for variations in run time and voltage. Z/X would invert ratio, X+Z or Z-X lack dimensional logic. Hence formula X divided by Z defines Rf.
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