Practice question
Question
Scatchard plot uses bound/free ligand on
Explanation
In the classic Scatchard representation the ratio of bound ligand to free ligand is plotted on the ordinate (y-axis) against the concentration of bound ligand on the abscissa. This arrangement yields a straight line whose slope equals –1/Kd and whose x-intercept equals total receptor number. Correct orientation of the axes is required for proper interpretation of affinity and capacity parameters.
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