Practice question
Question
Binding affinity is inversely proportional to
Explanation
The equilibrium dissociation constant Kd is the ratio of the dissociation rate constant to the association rate constant. A higher numerical value of Kd therefore indicates that dissociation is favored relative to association, resulting in weaker net binding. Affinity is consequently the reciprocal of Kd. This inverse relationship is fundamental to the quantitative interpretation of all reversible ligand–receptor interactions.