Practice question
Question
In SPR, the binding response is measured in:
Explanation
In Surface Plasmon Resonance instruments, interaction response is quantified as Resonance Units, abbreviated RU, where 1 RU approximately corresponds to binding of 1 picogram per square millimeter on sensor surface. RU reflects shift in resonance angle due to refractive index change proportional to bound mass. Real-time sensograms plot RU versus time, displaying association, equilibrium, and dissociation phases. Units such as nanometers, volts, or generic optical units are not used for SPR quantification. Calibration of RU allows calculation of stoichiometry, kinetics, and affinity constants with high sensitivity and reproducibility.
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