Practice question
Question
Which vector is best for cloning 200 kb DNA?
Explanation
Cloning capacity is determined by vector structure and host tolerance for large inserts. Plasmids handle up to 10 kb, lambda phage 20 kb, cosmid around 45 kb, BAC 100-300 kb, YAC 100-2000 kb and mammalian artificial chromosome exceeds 2000 kb. For 200 kb genomic fragments, YAC is optimal among common options because it includes centromere, telomeres and ARS enabling segregation of very large linear eukaryotic DNA in yeast. BAC can accommodate up to 300 kb but 200 kb near upper limit with lower stability. YAC accommodates megabase inserts essential for genome mapping projects.
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