Practice question

Poly(A) profiling is a 3′-end focused transcriptomic method that captures and sequences regions adjacent to polyadenylation sites. By using oligo(dT)-based enrichment and sequencing of the 3′ termini, it identifies alternative polyadenylation events, novel transcripts defined by distinct 3′ ends, and can reveal 3′ UTR alterations associated with gene fusions. Stating that it analyzes 5′ ends misrepresents its design, since 5′ cap analysis is performed by CAGE, not poly(A) profiling. Therefore the claim of 5′ end analysis is inconsistent with its mechanistic foundation and primary applications.