Practice question

Small RNA sequencing is a tailored RNA-seq protocol focusing on 18 to 35 nucleotide regulatory RNAs isolated by gel size selection after adapter ligation. It specifically profiles microRNAs, endogenous siRNAs, piRNAs and other short non-coding RNAs that regulate gene silencing, translation and chromatin modification. Whole transcriptome sequencing, WTS, captures all RNAs, mRNA-seq enriches polyadenylated long coding transcripts, and targeted RNA-seq interrogates specific panels. Only small RNA-seq enriches and quantifies microRNAs and siRNAs, resolving length variants, isomiRs and differential expression of these regulatory molecules.