Practice question

Time-resolved ChIP after inducing chromosomal break distinguishes persistent versus dynamic repair factor recruitment. Some proteins peak transiently then dissociate, others spread outward. Constitutive binding indicates stable occupancy at break site across all examined intervals without significant decline. In described experimental dataset, enrichment profiles show Rep3 maintaining high occupancy at break site from initial time point through later points, suggesting role as scaffold or structural maintenance component anchoring repair complex. This contrasts with Rep1 transient recruitment and Rep2 spreading phenotype, establishing Rep3 as constitutive binder at break.