Practice question

After analyte binding reaches steady state in Surface Plasmon Resonance cycle, regeneration step is essential to remove bound analyte while preserving immobilized ligand activity for subsequent cycles. Mild acidic, basic, high salt, or detergent solutions disrupt analyte-ligand interaction without denaturing ligand coating. Successful regeneration restores baseline resonance units, enabling replicate measurements and concentration series on same chip. Immobilization occurs before binding, whereas denaturation or dissolution would destroy sensor functionality. Regeneration conditions require optimization to balance complete removal and ligand stability across multiple analytical cycles.