Practice question

RNA isolation requires potent RNase inhibitors and denaturants such as GITC which chaotropically inactivates RNases, and phenol-chloroform which partitions RNA into aqueous phase while denaturing proteins. Chloroform aids phase separation and removes lipids. SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, is an analytical method for separating proteins by size based on SDS-denatured migration, not an extraction method for RNA. It is used post-extraction for protein analysis and would degrade RNA preparations. Thus SDS-PAGE has no role in RNA isolation workflow and is unrelated.