Practice question
Question
Which blotting method is used to detect DNA?
Explanation
Southern blotting, invented by Edwin Southern in 1975, remains the definitive method for detection of specific DNA sequences. Genomic DNA is fragmented with restriction endonucleases, resolved by agarose gel electrophoresis according to size, denatured under alkaline conditions to single strands, and capillary transferred to nitrocellulose or nylon membrane. Immobilized fragments are hybridized with radioactively or non-radioactively labeled complementary probes. Detection reveals fragment length polymorphism, enabling genotyping, DNA fingerprinting, transgene confirmation, and analysis of gene rearrangements with high specificity and reproducibility.
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