Practice question

Ion Torrent platform is frequently termed pH-mediated or semiconductor sequencing because its detection is based on hydrogen ions liberated during polymerization. The sensor chip contains millions of ion-sensitive field-effect transistors that detect small pH shifts rather than fluorescence. Nanopore sequencing measures ionic current blockade as DNA traverses protein pores, SBS denotes sequencing-by-synthesis with fluorescent reversible terminators used by Illumina, and tandem sequencing is not a standard term. Consequently, pH-mediated sequencing accurately describes Ion Torrent chemistry and distinguishes it from optical or nanopore methods.