Practice question
Question
In ELISA, which enzyme-substrate pair emits light instead of producing a color change?
Explanation
Luciferase oxidizes luciferin in presence of ATP and oxygen to produce bioluminescence, emitting photons quantified by luminometer rather than colorimetric absorbance. This chemiluminescent system generates light through excited state decay, offering high sensitivity with low background. Horseradish peroxidase with TMB and alkaline phosphatase with pNPP create colored products for spectrophotometric reading. Green fluorescent protein fluoresces intrinsically but lacks enzymatic turnover. Hence luciferin-based detection represents true light emission, widely applied in reporter assays, high sensitivity ELISA variants and ATP quantification improving dynamic range substantially.
Related blogs
Guides and insights connected to this topic
SARS-CoV-2 Coronavirus: Structure, Genome, Proteins, Replication, Pathogenesis, and Host Interaction
Coronavirus Overview Coronaviruses (CoVs) are enveloped viruses (surrounded by a lipid membrane derived from host cells), belonging to the s...
Coronavirus Structure, Genome, Replication, Symptoms, Pathogenesis, and Prevention
Structure of Coronavirus Coronaviruses (enveloped RNA viruses) fall in the virus family Coronaviridae, order Nidovirales. They are enveloped...
Cucumber Mosaic Virus (CMV): Structure, Genome, Symptoms, Disease Cycle and Management
Cucumber Mosaic Virus (CMV): Introduction and Importance Cucumber Mosaic Virus (CMV) (plant-infecting RNA virus) is one of the most economic...