Practice question

When a heteroduplex containing a mismatched restriction site undergoes mismatch repair, outcome depends on repair efficiency and strand bias. With complete repair, all molecules regain the restriction site and show uniform digestion pattern after electrophoresis. With no repair, all retain mismatch and show parental uncut pattern. At fifty percent efficiency, population becomes heterogeneous: half molecules are repaired and cleavable, half remain resistant. Gel then displays both parental and repaired bands simultaneously, reflecting mixed population important for interpreting heteroduplex analysis, mutagenesis, and gene conversion experiments.