Practice question

Cell counting for seeding uses dilution calculation C1V1 equals C2V2. Starting concentration 2.7 times ten to six cells per ml divided 27 fold yields 1 times ten to five cells per ml. Seeding 100 microliters equals 0.1 ml per well. Multiplying concentration by volume gives cells per well: 1 times ten to five times 0.1 equals 1 times ten to four. Earlier stated 2.7 times ten to four would be intermediate before final volume correction. Final count accounts for both dilution factor and aliquot volume ensuring accurate plating density.