Practice question

ChIP denotes Chromatin Immunoprecipitation, a cornerstone technique in epigenetics and transcriptional regulation. Chromatin consists of genomic DNA wrapped around octameric histone proteins forming nucleosomes with linker histones and non-histone regulators. Method involves formaldehyde crosslinking of living cells, chromatin fragmentation, selective immunoprecipitation using antibodies against transcription factors, coactivators or specific histone modifications like H3K4 trimethylation. Enriched DNA reveals promoters or enhancers occupied in vivo. Other expansions such as chromosomal insertion probe are terminologically incorrect and unrelated to immunoprecipitation chemistry taught for NEET and CSIR-NET examinations.