Practice question

Bimolecular Fluorescence Complementation is a genetic technique to visualize protein-protein association in living cells. Candidate proteins are fused to non-fluorescent N- and C-terminal fragments of a fluorescent protein such as YFP or Venus. Individually fragments are non-fluorescent. When fused proteins interact, fragments are brought into proximity to refold into functional fluorophore, emitting fluorescence detectable by microscopy or flow cytometry. Irreversible complementation reports localization of interaction. It does not measure RNA cleavage, phosphorylation per se, or mismatch repair but specifically protein-protein interaction.