Practice question

Amplified fragment length polymorphism combines advantages of restriction digestion and PCR amplification. Genomic DNA is first cut with two restriction enzymes, typically EcoRI and MseI, and adapters are ligated to sticky ends. Subsequent pre-selective and selective PCR using adapter-complementary primers with additional nucleotides amplifies a subset of fragments. This generates highly reproducible, multilocus fingerprints without prior sequence knowledge. RAPD uses only random PCR, SSR uses only locus-specific PCR, and SNP genotyping detects single base variations, lacking this dual enzymatic and amplification workflow.