Practice question

Fluorescence In Situ Hybridization is based on nucleic acid hybridization principle. A denatured target chromosomal DNA is annealed with complementary DNA or RNA probes directly conjugated or hapten-labeled for fluorochrome detection via avidin or secondary reagents. Visualization requires fluorescence microscopy, and stringent post-hybridization washes remove non-specifically bound probes to reduce background. Fluorescence-tagged antibodies are characteristic of immunofluorescence or immunohistochemistry for proteins, not essential for standard FISH chemistry. Although indirect FISH may use antibody-mediated detection layers, antibodies are not an obligate component of the core technique.