Practice question
Question
Which is a selectable marker in yeast artificial chromosome (YAC)?
Explanation
Yeast artificial chromosomes require selectable markers functional in auxotrophic yeast strains to ensure maintenance. URA3 encodes orotidine-5'-phosphate decarboxylase involved in uracil biosynthesis, complementing ura3 mutant phenotype. Yeast lacking functional URA3 cannot grow without uracil, so only transformants harboring YAC with URA3 survive on minimal medium. Similar markers include TRP1 for tryptophan and LEU2 for leucine selection. URA3 also allows counter-selection with 5-fluoroorotic acid for manipulating constructs. Bacterial markers like ampR are not selectable in yeast, making eukaryotic auxotrophic markers indispensable.
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