Practice question

Tm = 4(G+C) + 2(A+T) is obtained by applying the relevant formula or quantitative relationship to the given parameters. In DNA, numerical problem-solving requires understanding the mathematical relationships between biological variables. The calculation involves substituting the provided values into the appropriate equation and solving systematically. The other options (Tm = 2(A+T) + 4(G+C), Tm = 4(A+T) + 2(G+C), and Tm = 2(G+C) + 4(A+T)) result from common calculation errors such as using incorrect formulas, misidentifying variables, inverting ratios, or making arithmetic mistakes.