Practice question

When left and right circularly polarized beams pass chiral sample, slight difference in absorbance ΔA creates elliptically polarized transmitted light. Ellipticity theta quantifies degree of ellipticity, derived from ΔA using conversion factor involving natural log and degrees to radians. Expression theta degrees equals 2.303 times difference in absorbance of left and right components multiplied by 180 divided by 4π, approximately 32.98 times ΔA, usually reported as millidegrees. Molar ellipticity normalizes by concentration, path length and residue number for protein comparison.