Latex Test For C-reactive Protein

Submerged Fermentation.....

03 May 1052 views

Principle

This test based on the immunologic reaction between CRP as an antigen and latex particles have been coated with monospecific anti-human CRP and sensitized to detect levels greater than 6 μg/mL CRP.

The latex slide test has the advantage of rapid performance in comparison to other tests for detection of CRP.

Procedure

A. Quantitative Slide Test

Bring all reagents as well as the sample at room temperature (+15 °C to +30 °C).

Test serum has to be used undiluted.

Using the disposable plastic dropper, place one drop of test serum within the circled area on the special slide provided in the kit.

Add one drop of CRP Latex Reagent (shake the vial gently immediately before use) to the above drop and mix well with a disposable applicator stick & spread out to the edge of the test area.

Rock the slide gently to and fro for 2 minutes and examine for macroscopic agglutination under direct light source Do not examine beyond 2 minutes.

B. Semi-qualitative Slide Test

Prepare a series of dilutions of the test serum in normal saline e.g. 1:2, 1:4, 1:8 etc.

One drop of each of these dilutions is tested with one drop of CRP Latex Reagent.

Observe agglutination for no longer than 2 minutes on glass slide provided in the kit. The highest dilution which shows agglutination is taken as CRP titer of the test serum.

Clinical Significance

C-Reactive Protein (CRP) is an acute-phase protein found in concentrations of up to 5μg / mL in the serum of healthy persons. However, during an inflammatory response or infection, the levels may increase by as much as one thousand fold.

This increase in CRP level may be detected as early as 5 10 hours after tissue damage. The increase of CRP levels in serum appears to be a nonspecific phenomenon but the change can be used to monitor the course of certain diseases and their treatment.

The detection of CRP is more sensitive and reliable indicator of inflammatory processes than the erythrocyte sedimentation rate (ESR). The changes in concentration of CRP occur more rapidly than in the (ESR), elevated level of CRP can usually be demonstrated in cases of acute myocardial infarction, rheumatoid arthritis, bacterial and viral infections, acute rheumatic fever with or without carditis and in several types of malignancies particularly those with metastasis.

Many CRP latex kits suffer both from interference by rheumatoid factor and from prozone effects, but our CRP antigen has been specially formulated to minimize these problems.