(A) HSA, γ - globulin, RNAase and HEL

(B) HEL, RNAase, γ-globulin, HAS

(C) HEL, γ-globulin, RNAase, HSA

(D) HSA, RNAase, HEL, γ – globulin

Solution

1. At pH 7.0:

• HSA (pI = 4.9) and γ-globulin (pI = 6.6) will have a net negative charge and bind to the CM cellulose resin.
• RNAase (pI = 7.8) and HEL (pI = 11.0) will have a net positive charge and will not bind to the resin.

2. The order of elution will be:

• HSA will elute first, as it has the lowest pI and the weakest interaction with the resin. γ-globulin will elute next, as it has a higher pI and stronger interaction with the resin. RNAase and HEL will not bind to the resin

• st pI and the weakest interaction with the resin.
• γ-globulin will elute next, as it has a higher pI and stronger interaction with the resin.
• RNAase and HEL will not bind to the resin  and will elute together