- (A) 2 fragments
- (B) 3 fragments
- (C) 4 fragments
- (D) No clevage
Solutions
Chymotrypsin
cleaves peptide bonds on the carboxyl side of aromatic amino acids
(phenylalanine, tryptophan, and tyrosine) and large hydrophobic amino acids
(leucine, methionine, and histidine).
Given
peptide sequence: Gly-Arg-Ala-Ser-Phe-Gly-Asn-Lys-Trp-Glu
ValChymotrypsin
cleavage sites:
- 1. After
Phe (Phe-Gly)
- 2. After
Trp (Trp-Glu)
The
peptide will be cleaved into 3 fragments:
- 1.
Gly-Arg-Ala-Ser
- 2.
Phe-Gly-Asn-Lys
- 3.
Trp-Glu-Val
Therefore,
the correct answer is option (B)
Comments