- (A) salting out of DNA - denaturation
of protein.
- (B) salting out of protein -
denaturation of DNA.
- (C) denaturation of protein - salting
out of DNA.
- (D) denaturation of protein - salting
out of protei .
Solution
SDS: Denaturation
of protein
Sodium
dodecyl sulfate (SDS) is used to denature the proteins present in the sample.
SDS is a detergent that disrupts the hydrophobic interactions between amino acids
in the protein, causing the protein to unfold and lose its native structure.
This denaturation helps to remove proteins that might interfere with the purification
of DNA.
NaCl: Salting
out of proteins
Sodium
chloride (NaCl) is used to precipitate the proteins by increasing the ionic
strength of the solution. This process is known as salting out. The high
concentration of salt ions in the solution disrupts the solubility of proteins,
causing them to aggregate and precipitate out of the solution.
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