EXAMINATION OF LIVING BACTERIA : Hanging Drop Technique
IntroductionThe hanging drop technique is a method used in microbiology and cell biology to observe the behavior of living microorganisms or...
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Determination of KLa
IntroductionThe volumetric liquid mass transfer coefficient (KLa) is a useful parameter to characterize the bioreactor's capacity for aerati...
Electrophoretic separation of protein by SDS-PAGE
IntroductionElectrophoresis is the process of migration of charged molecules in response to an electric field. The rate of migration depends...
TRUST (Toludine Red Unheated Serum Test) For Syphilis
Principle The TRUST reagent containing modified VDRL antigen and toludine red pigment particles, flocclulate when mixed with sample containi...
RPR (Rapid Plasma Reagin ) test for Syphilis
Principle The RPR reagent containing modified VDRL antigen and microparticulate carbon particles, flocculate when mixed with sample containi...
Latex Test For C-reactive Protein
Principle This test based on the immunologic reaction between CRP as an antigen and latex particles have been coated with monospecific anti-...
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LATEX TEST FOR RHEUMATOID ARTHRITIS [RHEUMATOID FACTOR]
Principle Rheumatoid factor latex test kit consists of polystyrene latex particle coated with specially modified preparation of human gammag...
LATEX TEST FOR ANTI STREPTOLYSIN "O" (ASO)
Principle Antistreptolysin 'O' (ASO) test kit is based on the following principle Polystyrene latex particles are coated with purified and s...
ELISA Detection of HBsAg
Introduction Hepatitis B or Serum Hepatitis is one of the most common infectious diseases in the world and has become a global public health...
RNA estimation by Orcinol method
Introduction The colorimetric method suitable for pentose determination have been utilize for measuring RNA and involve reaction with orcino...
DNA Estimation by Diphenylamine (DPA) Method
IntroductionDetermining DNA quantity and purity is important prior to other reactions (PCR) for which it is necessary to know the exact DNA...
Isolation of genomic DNA from fungi.
Introduction Fungal genomes are among the smallest genomes of eukaryotes. The sizes of fungal genomes range from less than 10 Mbp to hundred...