Preparation of Bacteriological Growth Media
Nutrient AgarPrinciple Nutrient agar allow the growth of a variety of microorganisms that do not usually require specific nutrients or suppl...
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Latex Test For C-reactive Protein
Principle This test based on the immunologic reaction between CRP as an antigen and latex particles have been coated with monospecific anti-...
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LATEX TEST FOR RHEUMATOID ARTHRITIS [RHEUMATOID FACTOR]
Principle Rheumatoid factor latex test kit consists of polystyrene latex particle coated with specially modified preparation of human gammag...
LATEX TEST FOR ANTI STREPTOLYSIN "O" (ASO)
Principle Antistreptolysin 'O' (ASO) test kit is based on the following principle Polystyrene latex particles are coated with purified and s...
RNA estimation by Orcinol method
Introduction The colorimetric method suitable for pentose determination have been utilize for measuring RNA and involve reaction with orcino...
DNA Estimation by Diphenylamine (DPA) Method
IntroductionDetermining DNA quantity and purity is important prior to other reactions (PCR) for which it is necessary to know the exact DNA...
Isolation of genomic DNA from fungi.
Introduction Fungal genomes are among the smallest genomes of eukaryotes. The sizes of fungal genomes range from less than 10 Mbp to hundred...
Determination of Biochemical Oxygen Demand (BOD)
IntroductionBiochemical oxygen demand or BOD is a chemical procedure for determining the uptake rate of dissolved oxygen by the biological o...
Determination of TOTAL DISSOLVE SOLID (TDS)
IntroductionThe term “solids” is generally used when referring to any material suspended or dissolved in water or wastewater that can be phy...
Giemsa Stain- Principle, Procedure, Results, Interpretation
Introduction Giemsa stain was a name adopted from a Germany Chemist scientist, for his application of a combination of reagents in demonstra...
DNA Sequencing- Definition, Principle, Steps, Types, Uses
IntroductionsDNA sequencing refers to methods for determining the order of the nucleotide bases adenine,guanine,cytosine, and thym...
DNA Microarray- Definition, Principle, Procedure, Types
DNA microarrays are solid supports, usually of glass or silicon, upon which DNA is attached in an organized, pre-determined grid fashio...