Important Question
1. What is the possible angle of largest cone of light (come from object) which can be obtained
using high power objective?
- a) 62°
- b) 134°
- c) 82°
- d) 150°
2. What is the refractive index of water, air and cedarwood oil respectively?
- a) 1.0, 1.33, 1.51
- b) 1.51, 1.33, 1.0
- c) 1.33, 1.0, 1.51
- d) 1.33, 1.51, 1.0
3. Which of the following is to be used to obtain a cone of light of about 134°?
- a) Air
- b) Water
- c) Cedarwood oil
- d) Any of the above
4. Two lines close together _ can be separated with an oil immersion objective and
numerical aperture of 1.4.
- a) 0.50 µ
- b) 0.25 µ
- c) 2500 nm
- d) None of the above
5. The the NA of the objective, the __ will be its resolving power.
- a) Greater, greater
- b) Smaller, smaller
- c) Greater, smaller
- d) Both a) and b)
6. Which of the following material contain refractive index approximately around 1.52?
- a) Sandalwood oil
- b) Crown oil
- c) Cedarwood oil
- d) All of the above
7. What do you mean by equivalent focus?
- a) Objective lens system produces a real image of the object of the same size as it is produced by a simple biconvex lens
- b) Objective lens system produces a real image of the object of the same size as it is produced by a simple biconvex or converging lens
- c) Objective lens system produces a real image of the object of the same size as it is produced by a converging lens
- d) Objective lens system produces a virtual image of the object of the same size as it is produced by a simple biconvex or converging lens
8. The working distance is always than the equivalent focus of _:
- a) Less, objective
- b) Greater, objective
- c) Less, ocular
- d) Greater, ocular
9. The working distance of objects may be easily determined by:
- a) Knowing the wavelength of light source and multiplying it with numerical aperture
- b) Noting the number of complete turns of the fine adjustment required to bring object in sharp focus
- c) Noting the number of complete turns of the coarse adjustment required to bring object in sharp focus.
- d) All of the above
10. Each turn of micrometer screw (fine adjustment) raise the objective about _:
- a) 1 mm
- b) 0.1 mm
- c) 10 mm
- d) 6 mm
11. If the object is covered with a cover sleep, the working distance will __:
- a) Increase
- b) Decrease
- c) Will not change
- d) None of the above
12. What is correct for cover slip placed on object present on the glass slide?
- a) If cover slip is thicker than working distance of the objective, it will not become possible to get object in focus
- b) If the cover slip is thin, it will be possible to get the object in focus
- c) Focus of the microscope of the object covered with cover glass will be different from that of the uncovered object
- d) All of the above
13. The the refraction of the covering body (cover slip), the __ will be the apparent elevation:
- a) Greater, more
- b) Greater, less
- c) Lesser, more
- d) None of the above
14. How will you calculate the apparent depth of the object below the surface of cover glass?
- a) Index of refraction of cover glass
- b) Reciprocal of index of cover glass
- c) Index of refraction of cover glass multiplied by light wavelength
- d) Total turns of micrometer screw to bring object in sharp focus
Answer
| Qus | Ans | Qus | Ans |
| 1 | c | 8 | a |
| 2 | c | 9 | b |
| 3 | c | 10 | b |
| 4 | b | 11 | b |
| 5 | d | 12 | d |
| 6 | d | 13 | a |
| 7 | b | 14 | b |