MUST READ BEFORE CONTENT
- Bold option is answer of question.
- Preparation for Various Entrance Exams
- Support for college / university Exams
- All Doubts Clarification
1. What is the function of exciter filter?
- a) To excite light rays passes through it
- b) To remove light rays with larger wavelength
- c) To convert short wavelength rays into large wavelength rays
- d) To produce ultraviolet rays
2. The barrier filter will remove _ rays & only allow passing ___ rays:
- a) UV, Visible
- b) Visible, UV
- c) UV, Infrared
- d) Infrared, Visible
3. When any molecule absorbs radiant energy, it will become excited & release trapped energy as light. Which statement is true for this light?
- a) The light will have shorter wavelength than absorbed wavelength
- b) The light will have longer wavelength than absorbed wavelength
- c) The light will have equal wavelength to that of absorbed wavelength
- d) None of the above
4. Which light is used for illumination in fluorescence microscope?
- a) Violet light
- b) Blue light
- c) UV light
- d) All of the above
5. What is the other common name of epifluorescence microscopy?
- a) Incident light fluorescence microscopy
- b) Reflected fluorescence microscopy
- c) Both of the above
- d) Accidental fluorescence microscopy
6. What is the common most type of fluorescence microscopy?
- a) Incident light fluorescence microscopy
- b) Refraction fluorescence microscopy
- c) Both of the above
- d) None of the above
7. Which source is used to create illumination in fluorescence microscope?
- a) Tungsten filament
- b) White light bulb
- c) Mercury vapor arc lamp
- d) Plane polarized light
8. Name the mirror, used to direct light to microscope in fluorescence microscopy:
- a) Chromic mirror
- b) Dichromic mirror
- c) Chromatic mirror
- d) Dichromatic mirror
9. The special type of dye molecules used in fluorescence microscope are called:
- a) Auxochromes
- b) Fluorochromes
- c) Chromophores
- d) Fluorescents
10. Which of the following is not an example of fluorochromes?
- a) Diamino -1- phenyl indole
- b) Acridine orange
- c) Fluorescein isothiocynate (FITC)
- d) Rhodamine (tetramethyl rhodamine isothiocynate – TRITC)
11. Which of the following is not an example of fluorochromes?
- a) Acridine orange
- b) Diamidino-2-phenyl indole (DAPI)
- c) Fluorescein isothiocynate (FITC)
- d) Tetraphenoxyethyl rhodamine isothiocynate
12. Which of the following task is possible with fluorescence microscope?
- a) Photosynthetic microbes can be seen without applying fluorochromes
- b) Differentiation of live bacteria from dead bacteria
- c) Pathogens can be observed through antibodies/probes labeled with fluorochromes
- d) All of the above
13. How can we label microbes to view in fluorescence microscope?
- a) Fluorochromes labeled antibodies
- b) Fluorochromes labeled probes
- c) Fluorochromes labeled cell constituents
- d) All of the above
14. Which dye is selectively used to stain Mycobacterium species in a modified acid fast technique?
- a) Diamidino-2-phenyl indole (DAPI)
- b) Fluorescein isothiocynate
- c) Auramine
- d) None of the above
15. Which of the following fluorochrome selectively stains DNA?
- a) Diamidino-2-phenyl indole (DAPI)
- b) Rhodamine
- c) Acridine orange
- d) Both a & c
16. What is a measure of refractive index?
- a) Light passes from one medium to another
- b) A substance slows the velocity of light, direction &magnitude
- c) Proportion at which the ray will disappear
- d) The focus of light to form images
17. Epifluorescence microscope is used to visualize:
- a) Live microbial cells
- b) dead microbial cells
- c) Motile microbial cells
- d) Photosynthetic microbial cells
18. Barrier filter & exciter filter are parts of:
- a) Confocal scanning laser microscope
- b) Phase contrast microscope
- c) Fluorescence microscope
- d) Differential interference contrast microscope
19. Who is known as father of microscopy?
- a) Leeuwenhoek
- b) Robert Koch
- c) Louis Pasteur
- d) Robert Hooke
20. Why dichromatic mirror is used in fluorescence microscopy?
- a) It reflects shorter wavelength light to specimen & allow light of longer wavelength to pass through
- b) It produces 2 chromatic images of the object
- c) There are two mirrors to produce chromatic light
- d) It reflects longer wavelength light to microscope & allow light of shorter wavelength to pass through
21. Why some molecules illuminate on absorption of radiant energy?
- a) They become stable &release light
- b) They become excited &release trapped energy as light
- c) They become excited &release stable energy as light
- d) None of the above
22. Who won 2008 Nobel Prize in chemistry?
- a) Osamu Shimomura
- b) Martin Chalfie
- c) Roger Tsien
- d) All of the above
23. In which filed 2008 Nobel Prize was awarded to Osamu Shimomura, Martin Chalfie and Roger Tsien?
- a) Microbiology
- b) Physiology and medicine
- c) Chemistry
- d) Physics
24. The GFP gene was isolated from jellyfish belongs to genus :
- a) Aequorea
- b) Agaricus
- c) Gracilaria
- d) None of the above
25. What is the full name of GFP?
- a) Gray Fluorescent Protein
- b) Green Fluorescent Protein
- c) Gray Fluorescein Protein
- d) Green Fluorescein Protein
26. GFP gene is fused with the gene of interest and can be detected by _ microscopy:
- a) Confocal microscopy
- b) Phase contrast microscopy
- c) Florescence microscopy
- d) All of the above
27. Identify the correct match:
| Column A: Dye/s | Column B: Use |
| 1. Acridine orange and Diamidino-2-phenyl indole (DAPI) | i) Often attached to DNA probes or to antibodies that bind specific cellular components |
| 2. Fluorescein isothiocyanate (FITC) | ii) Often attached to antibodies that bind specific cellular components |
| 3. Tetramethyl rhodamine isothiocyanate (TRITC or rhodamine) | iii) Stains DNA |
- a) 1 and ii), 2 and iii), 3 and i)
- b) 1 and iii), 2 and i), 3 and ii)
- c) 1 and i), 2 and iii), 3 and ii)
- d) 1 and ii), 2 and i), 3 and iii)