1. What does the term Cryo mean?
- a) Sample preparation and visualization
- b) Cutting of sample
- c) Staining of sample
- d) Direct visualization of sample
2. What does the term Tomography mean?
- a) The method use to create ice crystals
- b) The method use to create images
- c) The method use to dry specimen
- d) None of the above
3. What is not correct for electron cryotomography?
- a) In this technique specimens are prepared by rapidly plunging in extremely cold liquid like ethane
- b) Rapid freezing forms ice crystals
- c) It preserves native structure and immobilizes the specimen
- d) The specimen is observed under the high vacuum
4. Identify the incorrect statement for electron cryotomography:
- a) In this technique the object is viewed from different angles, called tilt series
- b) The individual images are recorded and merged into a 3-dimensional structure
- c) The technique can be used to view magnetosomes and other cytoskeletal elements
- d) None of the above
5. Why specimen is rapidly frozen in electron cryotomography?
- a) Because it turns the specimen in powdery form
- b) Because it forms vitreous ice
- c) Because it forms ice crystals and immobilizes cell
- d) All of the above