MUST READ BEFORE CONTENT
- Bold option is answer of question.
- Preparation for Various Entrance Exams
- Support for college / university Exams
- All Doubts Clarification
1. The full form of CSLM is:
- a) Confocal Scanning Laser Microscope
- b) Contrast Scanning Laser Microscope
- c) Contrast Scanning Light Microscope
- d) Confocal Scanning Light Microscope
2. In CSLM, the light that forms an image comes from:
- a) The plane of focus
- b) The specimen above the focal plane
- c) The specimen below the focal plane
- d) All of the above
3. In CSLM the specimen can be:
- a) Illuminated with LED and can be stained by fluorescent dye
- b) Illuminated with laser beam and can be stained by fluorescent dye
- c) Illuminated with LED and can be stained by mutagenic dye
- d) Illuminated with laser beam and can be stained by mutagenic dye
4. In CSLM the aperture eliminates:
- a) Stray light from parts of the specimen that lie above and below the plane of focus
- b) Stray light from parts of the specimen that lie above the plane of focus
- c) Stray light from parts of the specimen that lie below the plane of focus
- d) Straight light from parts of the specimen that lie above and below the plane of focus
5. A computer interfaced with Confocal microscope shows different images:
- a) Digitized images from each plane in the specimen that is examined
- b) Digitized images from only one plane in the specimen that is examined
- c) Differential images from each plane in the specimen that is examined
- d) Digitized 3-D images from each plane in the specimen that is examined
6. Biofilms in medical field can be studied using:
- a) Epifluorescence microscopy
- b) Confocal scanning laser microscopy
- c) Phase contrast microscopy
- d) Differential interference contrast microscopy
7. What should be the approximate focal length (f) for CSLM?
- a) 16 mm
- b) 20 mm
- c) 50 mm
- d) 70 mm
8. CSLM Microscope uses one of the following combinations:
- a) Laser rays, Fluorescence staining and Computer
- b) Laser rays, Beams of positrons and Computer
- c) X-rays, Fluorescence staining and Probe
- d) X-rays, Scan pictures and Computer
9. What should be the approximate focal length (f) for CSLM?
- a) 16 mm
- b) 20 mm
- c) 50 mm
- d) 70 mm
10. What should be the approximate focal length (f) for CSLM?
- a) 16 mm
- b) 20 mm
- c) 50 mm
- d) 70 mm
11. The Confocal Microscope improves images in different ways:
- a) Illumination of one spot at a time reduces interference from light scattering by the rest of the specimen
- b) The aperture above the objective lens blocks out stray light
- c) Illumination of different spots at a time reduces interference from light by the rest of the specimen
- d) Both a and b
12. Confocal Scanning Laser Microscope is used to examine:
- a) Fluorescent stained specimen
- b) Mutagen stained specimen
- c) Cytological stained specimen
- d) Biochemical stained specimen
13. CSLM uses which one of the following combinations:
- a) Laser beam, fluorescence staining and computer
- b) Laser beam, positron beam and computer
- c) X ray, fluorescence staining and probe
- d) X ray, scan pictures and computers
14. How does CSLM improve images?
- a) Illumination of one spot at a time, reduces interference from light scattering by the rest of the specimen
- b) The aperture above the objective lens blocks out stray light
- c) Illumination of different spots at time reduces interference from light by the rest of the specimen
- d) Both a) and b)
15. CSLM is used to examine:
- a) Fluorescent stained specimen
- b) Mutagen stained specimen
- c) Cytological stained specimen
- d) Biochemical stained specimen
16. The major component of CSLM is:
- a) An aperture placed above the objective lens
- b) Above and below the plane of focus
- c) On the side of the plane of focus
- d) On to the plane of focus
17. CSLM is used to study ____:
- a) Biofilms on medical devices
- b) Colonization in parts of the body
- c) Biomass in muscle tissues
- d) None of the above
18. Identify the microscopy technique from below image:

- a) Phase-contrast microscope
- b) Dark-field microscope
- c) Simple microscope
- d) Electron microscope
19. What do you mean by “F” and “f” shown in the below image:

- a) F – focal length, f – focal point
- b) F – focal point, f – focal length
- c) F – final length, f – final point
- d) F – final point, f – final length
20. Out of figure A or figure B shown below, whose resolution will be higher?

- a) A
- b) B
- c) Similar in both
- d) Cannot decide from above image
21. Identify microscopy from below shown image:

- a) Phase-contrast microscope
- b) Dark-field microscope
- c) Simple microscope
- d) Electron microscope