MUST READ BEFORE CONTENT
- Bold option is answer of question.
- Preparation for Various Entrance Exams
- Support for college / university Exams
- All Doubts Clarification
1. Why ordinary microscope is called bright field microscope?
- a) It forms a brighter image against dark background
- b) It forms a darker image against bright background
- c) It forms a brighter image in bright background
- d) It forms a darker image in dark background
2. Which one of the following is a part of bright field microscope?
- a) Arm
- b) Diaphragm
- c) Light source
- d) All of the above
3. What is the function of condenser?
- a) To create illumination
- b) To move stage
- c) To focus a cone of light on slide
- d) All of the above
4. The image should remain in focus when objective lenses are changed. This property of microscope is known as:
- a) Magnification
- b) Resolution
- c) Parfocal
- d) None of the above
5. The lens nearer to eye, when viewing in microscope is known as:
- a) Nose piece
- b) Ocular lens
- c) Objective lens
- d) Oil immersion lens
6. Minimal resolvable distance between two objects is equal to:
- a) 0.5ƛ/ɳ sin ɵ
- b) 0.5ƛ/ɳ cos ɵ
- c) 0.05ƛ/ɳ sin ɵ
- d) 0.05ƛ/ɳ cos ɵ
7. What is the approximate focal length for scanning objective?
- a) 20mm
- b) 40mm
- c) 10mm
- d) 4mm
8. What should be the working distance for high power objective?
- a) 0.5-0.7mm
- b) 0.7-0.9mm
- c) 0.9-1.0 mm
- d) 1.0-1.2 mm
9. The resolving power is limited by the __ of the illuminating beam.
- a) Frequency
- b) Velocity
- c) Speed
- d) Wavelength
10. What is the numerical aperture of low power objective?
- a) 0.65
- b) 0.25
- c) 1.25
- d) 0.10
11. To focus a specimen, it is best to start with which objective?
- a) High power
- b) Low power
- c) Scanning
- d) Oil immersion
12. For which of the following specimens would you use a dissecting scope?
- a) Human skin cells
- b) Insect mouth parts
- c) Escherichia coli
- d) Newspaper print.
13. Which objective provides the greatest field to view?
- a) This depends on if the specimen is stained
- b) Low power
- c) High power
- d) Oil immersion
14. Which one can act as a source of illumination for microscopes?
- a) Ultraviolet light
- b) Sunlight
- c) Visible light
- d) All of the above
15. Which equation represents resolution of an entire microscope?
- a) d/NAobjective + NAcondenser
- b) ƛ/NAobjective x NAcondenser
- c) ƛ/NAobjective + NAcondenser
- d) d/NAobjective x NAcondenser
16. What would be the resolving power of microscope with low power objective (NA 0.25) and 530 nm light wavelength?
- a) 212nm
- b) 1060nm
- c) 2120nm
- d) 4240nm
17. What would be the resolving power of microscope with high power objective (NA 0.65) and 550 nm light wavelength?
- a) 212nm
- b) 846nm
- c) 423nm
- d) 1692nm
18. Identify correct match of magnification, numerical aperture, focal length, working distance
criteria shown in below columns:
| Column A | Column B |
| 1. Oil immersion objective | i) 4X, 0.10, 40 mm, 20 mm |
| 2. High power objective | ii) 1000X, 1.25, 2 mm, 0.1 mm |
| 3. Low power objective | iii) 45X, 0.65, 4 mm, 0.7mm |
| 4. Scanning objective | iv) 10X, 0.25, 16mm, 8 mm |
- a) 1 and ii), 2 and iv), 3 and i), 4 and iii)
- b) 1 and ii), 2 and iii), 3 and iv), 4 and i)
- c) 1 and iii), 2 and i), 3 and ii), 4 and iv)
- d) 1 and iv), 2 and iii), 3 and ii), 4 and i)
19. What do you mean by binocular microscope?
- a) Contains 2 eyepieces
- b) Contains only 2 objectives
- c) Contains at least 2 objectives
- d) Contains 2 lenses in eyepiece
20. Which microscope can be used to view living microbes?
- a) Bright field microscope
- b) Fluorescence microscope
- c) Phase contrast microscope
- d) All of the above
21. One micrometer is equal to:
- a) 10-6 meter
- b) 10-3 meter
- c) 10-9 meter
- d) 10-10 meter
22. What does binocular microscope contain?
- a) Two oculars
- b) Two objectives
- c) One objective & one eye piece
- d) Two oil immersion lenses
23. Which one of the following is exact definition of parfocal?
- a) The image would be focused by fine adjustment
- b) The image would be focused by coarse adjustment
- c) Image should remain in focus, when objectives are changed
- d) Image should remain in focus, when oculars are changed
24. The image what we observe in microscope is created by:
- a) Objective
- b) Ocular
- c) Both a & b
- d) Either of a or b
25. How will you calculate total magnification of image?
- a) By making total of magnification of objective &eyepiece
- b) By making multiplication of magnification of objective &eyepiece
- c) By total of magnification of all objectives attached to nose piece
- d) By multiplication of magnification of all objectives attached to nose piece
26. If microscope contains 45x objective &5x eyepiece, the total magnification will be:
- a) 450x
- b) 225x
- c) 125x
- d) 50x
27. Which one of the following is exact definition of resolution?
- a) The ability of a lens to separate or distinguish between small objects those are far from each other
- b) The ability of a lens to separate or distinguish between small objects those are close together
- c) The ability of a lens to separate or distinguish between small objects that are overlapping each other
- d) None of the above
28. To obtain greatest possible resolution in bright field microscope one can use light with
wavelength:
- a) 450-500nm
- b) 500-550nm
- c) 550-600nm
- d) 700-800nm
29. The refractive index of air is:
- a) 0.1
- b) 1
- c) 10
- d) None of the above
30. The refractive index of glass &cedar wood oil is:
- a) About 1.1
- b) Differ about 0.5 from each other
- c) Nearer to each other
- d) 10 times higher than air
31. The refractive index of glass & cedar wood oil is:
- a) About 1.5 times higher than air
- b) Nearer to each other
- c) Average 1.53
- d) All of the above
32. The lens working in an air cannot have numerical aperture more than:
- a) 1.25
- b) 1.00
- c) 0.65
- d) 0.25
33. How can we increase numerical aperture more than 1?
- a) By using air
- b) By using immersion oil
- c) Both a & b
- d) None of the above
34. Which of the following are true magnification, numerical aperture, focal length & working
distance of criteria for an oil immersion lens?
- a) 45X, 0.65, 4mm, 0.7mm
- b) 100X, 1.25, 1.8mm, 0.1mm
- c) 100X, 0.25, 16mm, 8mm
- d) 4X, 0.10, 40mm, 20mm
35. Which of the following are true magnification, numerical aperture, focal length & working
distance of criteria for high power objective?
- a) 45X, 0.65, 4mm, 0.7mm
- b) 100X, 1.25, 1.8mm, 0.3mm
- c) 100X, 0.65, 16mm, 8mm
- d) 4X, 0.10, 60mm, 20mm
36. Which of the following are true magnification, numerical aperture, focal length & working
distance of criteria for low power objective?
- a) 45X, 0.25, 16mm, 0.7mm
- b) 100X, 1.25, 3.8mm, 0.1mm
- c) 10X, 0.25, 16mm, 8mm
- d) 4X, 0.10, 40mm, 30mm
37. In ɳsinɵ equation of numerical aperture, ɵ is:
- a) The angle of the cone of light entering in an objective
- b) 1/2angle of the cone of light entering in an objective
- c) 1/4 angle of the cone of light entering in an objective
- d) None of the above
38. In ɳsinɵ equation maximum value of ɵ is:
- a) 90°
- b) 45°
- c) 180°
- d) 360°
39. The maximum value of sinɵ is:
- a) 0.5
- b) 0.65
- c) 1.00
- d) 1.25
40. The working distance can be defined as the distance between:
- a) Objective &eyepiece
- b) 2 objectives
- c) Objective &condenser
- d) Objective &specimen on slide
41. Which of the following objective works on shortest working distance?
- a) Low power
- b) Scanning lens
- c) Oil immersion lens
- d) High power
42. The objective with large working distance will have:
- a) Small numerical aperture &low resolving power
- b) Large numerical aperture &high resolving power
- c) Large numerical aperture &low resolving power
- d) Small numerical aperture &high resolving power
43. The resolution of microscope depends on:
- a) Numerical aperture of objective
- b) Numerical aperture of condenser
- c) Wavelength of light
- d) All of the above
44. Microscope condenser contains numerical aperture about:
- a) 1.0
- b) 1.3
- c) 1.5
- d) 2.0
45. The maximum obtainable theoretical resolution of light microscope with oil immersion lens is:
- a) 212nm
- b) 312nm
- c) 112nm
- d) 252nm
46. What is the maximum obtainable theoretical resolution of light microscope with oil immersion lens?
- a) 0.2μm
- b) 0.3μm
- c) 0.02μm
- d) 0.03μm
47. A light microscope can maximum distinguishes between 2 dots about __μm apart:
- a) 0.3μm
- b) 0.2μm
- c) 0.1μm
- d) 0.4μm
48. can prepare bright field microscope with even 10,000X magnification, but it is not useful
because:
- a) It is costlier
- b) It would simply magnify blur
- c) Increase the amount of artifacts
- d) All of the above